Fig. 8.

PhiPA1-3 rescued the P. aeruginosa PA001-infected zebrafish. (A) Assessment of lethal dosage; three groups were considered, each containing eight fish. These were injected with PA001 cells (2 × 10⁸, black line; 2 × 10⁷, red line; 2 × 10⁶, green line; CFU/20 µL, respectively); (B) The disease symptoms of zebrafish infected with PA001, as compared to those of the phage-rescued group (MOI=10) and control (0.85% NaCl). P. aeruginosa PA001 was introduced first into the fish, followed by administration of phiPA1-3 (MOI=10) after 30 min. Left: side view; middle: top view; right: abdomen anatomy; (C) The survival rates of PA001-infected zebrafish were measured under different treatments: MIC-gentamicin (4 µg/mL, indicated by the green line), MIC-gentamicin alone (red line), phiPA1-3 treatment (MOI=10) after a 30 min interval following bacterial challenge (orange line), PA001 treatment alone (2 × 10⁷ CFU/20 µL, blue line), phiPA1-3 injection alone (black line), and 0.85% NaCl buffer as the negative control (purple line) The X-axis represents the time post-infection, while the Y-axis represents the survival percentage of the zebrafish. The significance of the differences between groups was assessed by means of the log-rank and Gehan–Breslow–Wilcoxon tests using GraphPad Prism 9. “****” indicates p<0.0001, “**” indicates p<0.001, and ns means no significance. MOI, multiplicity of infection.