FIG. 1.

Schematic display of plasmid-encoded full-length and defective genomes of CSFV. (A) Full-length genomes of A187-1 and A187-CAT. The 5′- and 3′UTRs are depicted as black lines, and the open reading frame is shown as a box composed of the individual viral genes encoding structural (hatched) and nonstructural (white) proteins as indicated. Vertical lines indicate the SrfI¹²²⁹⁸ and the NruI¹¹³⁰¹ sites which were used to linearize the respective plasmids prior to in vitro RNA transcription. The CAT marker gene inserted in A187-CAT at nt 386 of the genome is shown as a separate box (black). (B) Defective genomes. The position and extent of the individual deletions (dashed lines) are given in parentheses for each construct. Numbers refer to the nucleotide sequence of CSFV Alfort/187 (25). The ubiquitin gene is shown as a black box.