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. Author manuscript; available in PMC: 2024 Jun 5.
Published in final edited form as: Angew Chem Int Ed Engl. 2023 Apr 28;62(23):e202212636. doi: 10.1002/anie.202212636

Figure 5.

Figure 5.

Competition SPR corroborates ApoE’s recognition of 3-O-S in LMWHS Array. (A) Scheme for competition SPR. (B) SPR competition with chemically desulfated heparin. The inhibition of ApoE/Heparin interactions by chemically desulfated heparin indicates that the 6-O-S group plays a large role compared to the 2-O-S group. Whereas fully sulfated heparin almost completely abolishes ApoE3 binding to the heparin chip, 2-O-desulfated heparin retains about 60% ApoE binding, while 6-O-S desulfated heparin retains 80% binding and N-desulfated heparin retains 70%. (C) The 3-O-Sulfated Oligo-19 inhibits ApoE3-HS binding with an IC50 of 0.4 μM, compared to (D) oligo-20, which inhibits ApoE-HS binding with an IC50 of 2.2 μM. Error bars are ± SEM.