Figure 2.

Y220C-specificity of compounds involved in cell proliferation inhibition. (A) Viability of indicated cell lines expressing endogenous p53 mutant treated with B4, B5, B7, H3, H9, or PK083 for three days. Half-maximal inhibitory concentration (IC₅₀) values were calculated using non-linear curve fitting with GraphPad Prism 8.0. (B) Dots represent the calculated IC₅₀ values in cell lines with p53-Y220C or other mutations. (C) Mutant p53-Y220C in NUGC-3 and BxPC-3 cells was knocked out using the clustered regularly interspaced palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system, and viability was assessed using the cell viability assay. Error bars in (A, C) represent the mean ± SD. p values were calculated using two-tailed t-tests. *p < 0.05.