Table 1.
NOP mRNA expression in HUVECs. The presence of NOP receptor mRNA was detected in HUVECs treated for 24 h with 50% or 75% conditioned media from MCF-7 breast cancer cells or VEGF/FGF 10 ng ml−1 for 24 or 48 h. To determine ΔCT, the geometric mean of the housekeeper genes ELF2B1 and B2M was used. Data are presented as mean [standard error of the mean] of 32 assays (in 32 individual cell lines) for control and treated samples and 31 for 75% MCF-7 cells. There was no MOP receptor mRNA detected and in any cell line, and there was no amplification in any non-template control. There were no statistically significant differences (analysis of variance) amongst the groups. FGF, fibroblast growth factor; HUVEC, human umbilical vein endothelial cell; MCF-7, Michigan Cancer Foundation-7; NOP, naloxone-insensitive Nociceptin/Orphanin FQ peptide receptor; VEGF, vascular endothelial growth factor.
| Condition | HK-CT(GEOM) | NOP-CT | ΔCT |
|---|---|---|---|
| Control | 25.43 [0.35] | 32.03 [0.49] | 6.60 [0.55] |
| MCF-7 media 50% (24h) | 25.31 [0.47] | 31.60 [0.49] | 6.29 [0.40] |
| MCF-7 media 75% (24h) | 25.78 [0.40] | 31.45 [0.45] | 5.67 [0.44] |
| VEGF/FGF 10ng ml−1 (24h) | 24.38 [0.36] | 31.73 [0.46] | 7.35 [0.53] |
| VEGF/FGF 10ng ml−1 (48h) | 25.22 [0.53] | 31.47 [0.47] | 6.25 [0.36] |