FIG. 3.

In vitro HDVagrz activity. (A) Two percent agarose gel with RNA transcribed from ca. 1,300- and 1,400-bp PCR products obtained from the 3′ proximal fragments of pHST18 (lane 18) or pHST20 (lane 20), respectively (see Fig. 1 for the locations of primer annealing sites). The upper band represents uncleaved RNA, and the lower bands in the lane with RNA from pHST20 (lane 20) represent the HDVagrz cleavage products. The positions of double-stranded DNA size markers are indicated in basepairs. (B) RNA hybridization assay with pHS49 (24) as a specific probe for detection of 3′ proximal TBSV RNA sequences. (C) RNA hybridization of a similar Northern blot with p3′NT (28) as a probe for detection of nos-poly(A) sequences.