Skip to main content
. Author manuscript; available in PMC: 2024 Feb 1.
Published in final edited form as: Kidney Int. 2022 Oct 28;103(2):331–342. doi: 10.1016/j.kint.2022.09.025

Figure 10. Melanocortin 1 receptor (MC1R) is expressed by diverse subsets of renal leukocytes and inversely correlates with the nuclear factor (NFκB) pathway.

Figure 10.

(a) Flow cytometry analysis of renal leukocytes isolated from wild-type (WT) mice with nephrotoxic serum (NTS) nephritis (pool of 3 animals per group) demonstrates MC1R expression in CD45+, F4/80+, CD3+, and CD19+ renal leukocytes. (b) Representative immunoblots show MC1R expression in bone marrow-derived macrophages and splenocyte-derived T and B cells prepared from WT or e/e (melanocortin 1 receptor–null) mice. Asterisks indicate nonspecific bands. (c) Gene set enrichment analysis based on data derived from the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) database (GEO accession number GSE121239) demonstrated that MC1R expression inversely correlates with the curated gene set “BIOCARTA_NFKB_PATHWAY” in peripheral blood mononuclear cells collected from patients with systemic lupus erythematosus. Normalized enrichment score (NES) and nominal P value are shown.