(a) Animals were treated as shown in Figure 1. Representative micrographs show fluorescent
immunohistochemistry staining of kidney specimens for indicated proteins, with
glomeruli outlined by dashed circles. (bar=20 μm). White arrowheads
indicate desmin expression in synaptopodin (SYNPO)-positive podocytes. (b)
Representative blots show immunoblot analysis of isolated glomeruli for
indicated proteins. (c, d) Estimation of the abundance of wilms’ tumor-1
(WT-1) and podocin expression by densitometric analyses of immunoblots,
presented as relative levels normalized to actin.
*P<0.05 by analysis of variance.
aP<0.01 versus e/e (MC1R-null) group
treated with NTS and Vehicle (Veh) gel or wild-type (WT) group with NTS and
repository corticotropin injection (RCI) treatment;
bP<0.01 versus e/e mice treated with NTS
and RCI; cP<0.05 versus e/e mice treated with
NTS and Veh or WT mice treated with NTS and pan-melanocortin receptor agonist
[Nle, DPhe]-α-MSH (NDP-MSH) or MC1R agonist MS05 (custom-made peptide, GL
Biochem); dP<;0.05 versus e/e mice treated
with NTS and NDP-MSH; (n=6). To optimize viewing of this image, please see the
online version of this article at www.kidney-international.org.