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. Author manuscript; available in PMC: 2024 Feb 1.
Published in final edited form as: Kidney Int. 2022 Oct 28;103(2):331–342. doi: 10.1016/j.kint.2022.09.025

Figure 6. Melanocortin 1 receptor (MC1R) deficiency increases kidney infiltration of macrophages in nephrotoxic serum (NTS) nephritis and enhances M1 polarization.

Figure 6.

(a) Representative micrographs show dual-color immunofluorescence staining of kidney specimens procured from IgG or NTS-injured and vehicle-treated mice for F4/80 and inducible nitric oxide synthase (iNOS) or mannose receptor (MR), with glomeruli outlined by dashed circles (bar=25 μm). Arrowheads indicate cells positive for dual-color staining. (b) Kidney homogenates were processed for immunoblot analysis for indicated proteins. (c) Estimation of the abundance of indicated proteins in NTS-injured groups by densitometric analyses of immunoblots, expressed as relative levels normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). aP<0.05 versus NTS-injured wild-type (WT) mice for the same protein by unpaired t test (n=4). To optimize viewing of this image, please see the online version of this article at www.kidney-international.org.