Figure 8. Loss of yeast DJ-1 members induces mitochondrial dysfunction.
(A) Visualization of mitochondrial morphology. WT and ∆Q strains expressing MTS-mCherry (decorates mitochondria) were either treated with buffer (-GO) or 15 mM GO (+GO), followed by imaging. (B, C) FACS analysis to estimate total and functional mitochondrial mass. Respective strains were grown till the early log phase, followed by incubation with GO. Later, the cells were stained with Nonyl Acridine Orange (NAO) for total mass and TetraMethylRhodamine ethyl ester (TMRE) for determining functional mass. (D) Measurement of ATP levels. Selected strains were exposed to GO treatment at the mid-log phase. Consequently, the mitochondria were isolated, and the ATP levels were estimated through a fluorescence assay. (E) mtDNA staining by SYTO18. Following the treatment with GO, WT, and ∆Q were stained with SYTO18 dye. The microscopic analysis was performed in a confocal microscope (Olympus FV3000), with10 µm scale in images. All experiments were performed in three independent biological replicates and analysed through paired t-test to determine significance, *, p≤0.05; **, p≤0.01; ***, p≤0.001; NS, not significant.
Figure 8—figure supplement 1. Phenotypic analysis of Hsp31 deletion strains in non-fermentable carbon source.
