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. 2023 Aug 7;12:e88875. doi: 10.7554/eLife.88875

Figure 8. Loss of yeast DJ-1 members induces mitochondrial dysfunction.

(A) Visualization of mitochondrial morphology. WT and ∆Q strains expressing MTS-mCherry (decorates mitochondria) were either treated with buffer (-GO) or 15 mM GO (+GO), followed by imaging. (B, C) FACS analysis to estimate total and functional mitochondrial mass. Respective strains were grown till the early log phase, followed by incubation with GO. Later, the cells were stained with Nonyl Acridine Orange (NAO) for total mass and TetraMethylRhodamine ethyl ester (TMRE) for determining functional mass. (D) Measurement of ATP levels. Selected strains were exposed to GO treatment at the mid-log phase. Consequently, the mitochondria were isolated, and the ATP levels were estimated through a fluorescence assay. (E) mtDNA staining by SYTO18. Following the treatment with GO, WT, and ∆Q were stained with SYTO18 dye. The microscopic analysis was performed in a confocal microscope (Olympus FV3000), with10 µm scale in images. All experiments were performed in three independent biological replicates and analysed through paired t-test to determine significance, *, p≤0.05; **, p≤0.01; ***, p≤0.001; NS, not significant.

Figure 8—source data 1. Source data for Figure 8B contains densitometric values for graph.
Figure 8—source data 2. Source data for Figure 8C contains densitometric values for graph.
Figure 8—source data 3. Source data for Figure 8D contains densitometric values for graph.

Figure 8.

Figure 8—figure supplement 1. Phenotypic analysis of Hsp31 deletion strains in non-fermentable carbon source.

Figure 8—figure supplement 1.

(A) Selected strains were grown till the early log phase and spotted on media plates containing YP dextrose (2%) or YP glycerol (2%). Plates were incubated at 30 °C, and images were acquired at 36 hr.