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. Author manuscript; available in PMC: 2024 May 8.
Published in final edited form as: Curr Biol. 2023 Apr 13;33(9):1825–1835.e3. doi: 10.1016/j.cub.2023.03.057

Figure 3 |. Y-linked piRNA loci are active and functional in both sexes, when inherited from either parent, regardless of whether mothers carry a Y chromosome.

Figure 3 |

(A) Top: Cross schemes that generate: XX females without mothers bearing a Y (column 1), XX females with Y-bearing mothers (column 2), XXY females without mothers bearing a Y (column 3), XXY females with Y-bearing mothers but inhering the Y from the father (column 4), and XXY females with Y-bearing mothers and inheriting the Y from the mother (column 5). Middle: cartoon showing the genotype of each kind of females generated and whether their mothers carry a Y is reflected by whether they receive maternally deposited Y-derived piRNAs. Bottom: RNA in situ HCR detecting Su(Ste) piRNA precursors (row 1), petrel piRNA precursors (row 2), and pirate mRNAs (row 3) in stage 6-7 egg chambers. Scale bar: 5μm.

(B) Top: Cross schemes that generate: XO males without mothers bearing a Y (column 1), XO males with Y-bearing mothers (column 2), XY males without mothers bearing a Y (column 3), XY males with Y-bearing mothers but inhering the Y from the father (column 4), and XY males with Y-bearing mothers and inheriting the Y from the mother (column 5). Middle: cartoon showing the genotype of each kind of males generated and whether their mothers carry a Y is reflected by whether they receive maternally deposited Y-derived piRNAs. Bottom: RNA in situ HCR detecting Su(Ste) piRNA precursors (row 1), Stellate mRNAs (row 2), petrel piRNA precursors (row 3), and pirate mRNAs (row 4) and at the apical tips of the testes. Scale bar: 10μm.