Extended Data Fig. 3. Activation of the UPRER in by 1-undecene odor does not require immune response regulators and occurs in RIM/RIC interneurons.
a, b, c, Representative fluorescence microscopy images and quantification of hsp-4p::GFP fluorescence in a, zip-2(ok3730), b, pmk-1(k25) and c, kgb-1(um3) animals with or without exposure to 1-undecene odor for 12 hours. Experiments were repeated three times. n = 50, 58 animals in a, n = 23, 18 animals in b and n = 54, 48 animals in c. Scale bars, 200 μm. Graphs show mean ± s.d. ***P < 0.001 in a and ****P < 0.0001 in b and c (two-tailed unpaired Student’s t test). d, Representative image of worms expressing a tdc-1p::mKate; xbp-1p::xbp-1::GFP transgene exposed to 8 hours of 1-undecene odor. Scale bars, 10 μm. Experiment repeated one time. Scale bars, 10 μm. e, Representative fluorescence microscopy images and quantification of hsp-4p::GFP fluorescence in hsp-4p::GFP animals with or without an unc-13(e450) mutant background grown from L1 larval stage on NGM plates containing bacteria harboring empty vector (L4440) or pdi-2 RNAi. Data were normalized by samples treated with vector only. The experiment was repeated twice. n = 11, 13, 11, 10. Scale bars, 200 μm. Graphs show mean ± s.d. **P < 0.01, ****P < 0.0001 (two-tailed unpaired Student’s t test). Source Data contains precise P values.