Extended Data Fig. 6. IgE-dependent and independent roles in gut allergic inflammation.
a, Experimental protocol for the development of gut allergic inflammation and systemic anaphylaxis. Allergic BALB/c WT and littermate IgE KO mice were sensitized with OVA/alum whereas control mice received alum alone. All mice were orally challenged with OVA. b, Rectal temperature over time (left) and maximum temperature variation (right) after systemic OVA challenge on d1 (n = 4 WT control and n = 5 allergic, IgE KO control and allergic). c, OVA-specific IgE (left) and IgG1 (right) serum antibodies after five oral challenges with OVA (n = 13-17 per group L, n = 5-9 per group R). d, Gastrointestinal transit time was determined on day 31 following OVA challenge (n = 7 WT controls, 10 WT allergic, 10 IgE KO controls, 8 IgE KO allergic). e, Systemic levels of corticosterone 1h after the fifth OVA challenge in control and OVA/alum sensitized mice (n = 14 WT controls and allergic, 5 IgE KO controls and allergic). f-g, Flow cytometry analysis of isolated small intestinal cells from the epithelial layer (left) and lamina propria (centre and right) (n = 8 WT control and allergic, 6 IgE KO control, 5 IgE KO allergic, f) (n = 11 WT control, 13 WT allergic, 10 IgE KO control, 7 IgE KO allergic, g). h, Flow cytometry analysis of enteric mast cells (CD45+ CD19- CD3- CD11b- CD117int FcεRI+ cells) from the small intestine of OVA/alum sensitized BALB/c WT mice after OVA challenges. Controls were sensitized with alum alone and challenged with oral OVA. Graphs show mean ± s.e.m. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. Two-tailed Mann-Whitney test. Each panel is representative of at least two independent experiments. a, Created with BioRender.com.