Extended Data Fig. 8. GDF15 is induced upon allergen exposure.
a, GDF15 serum levels induced by oral (left) (n = 3-8 control and 3-10 allergic per timepoint) or intravenous (right)(n = 4 control and 4 allergic per timepoint) OVA administration in sensitized BALB/c mice. b-d, Serum GDF15 induction after oral allergen challenges in OVA/alum sensitized BALB/c and C57BL/6 (n = 4 C57BL/6J, 9 BALB/cJ)(b), IgE KO (n = 9 WT, 15 IgE KO) (c), or mast cell-depleted RMB (n = 5-8 WT, 5-10 MC Dep mice per timepoint) (d) mice. e, Induction of serum GDF15 in control and sensitized mice after the administration of the 5- lipoxygenase inhibitor zileuton during oral challenges with OVA (n = 5 control vehicle, 5 allergic vehicle, 5 control zileuton, 4 allergic zileuton). f, Fold change in Gdf15 mRNA transcripts in the intestinal tissues of OVA/alum sensitized BALB/c mice relative to control groups (n = 9 WT allergic normalized to the average transcripts of 7 WT control mice per tissue). g, Gdf15 mRNA transcripts in the duodenum (left) and colon (right) in sensitized mice (n = 7 WT control, 9 WT allergic, 6 IgE KO allergic, 5 MC Dep allergic). Graphs show mean ± s.e.m. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. a-d, Two-tailed Student’s T-test, e, Two-tailed Mann-Whitney test, g, One-way ANOVA with Dunett’s multiple comparisons test. Each panel is representative of at least two independent experiments.