Extended Data Fig. 1. Characterization of immunological avoidance.
a, Cumulative licks of water solution from right- or left-positioned bottles from control (left) and OVA/alum sensitized BALB/c mice (right) (n = 15-18). b, Preference to the water bottle located on the right side by control and OVA/alum sensitized mice (n = 15 control and 17 allergic). c, Solution intake of water and varying concentrations of OVA in control mice on day 1 of the two-bottle preference test (n = 6 per concentration). d, Cumulative licks to different concentrations of OVA from mice sensitized with OVA/alum (n = 5-6 per concentration). e, Preference to different concentrations of OVA in sensitized mice (n = 4-6 per concentration). f, Total number of licks during water baseline or OVA test day 1, determined as the sum of licks from the two bottles at 200 min (n = 12-13 control and 15-16 allergic). g, Preference to 1% OVA solution within 10 min of offering the two bottles (n = 16 control and 18 allergic). h, OVA preference by OVA/alum sensitized TRPM5 WT or KO mice (n = 5 WT and 6 TRPM5 KO). i, Experimental protocol for oral sensitization to OVA with cholera toxin (CT). Mice were administered with i.g. OVA with or without CT on days 0 and 7. Controls received OVA alone. j, Preference to OVA on day 1 of preference test (n = 5 control and 5 CT sensitized). k-m, Total number of cFos+ cells in the area postrema (AP), lateral hypothalamus (LH), and paraventricular nucleus of the hypothalamus (PVN) of OVA/alum sensitized mice (n = 6 control and 6 allergic). Graphs show mean ± s.e.m. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. a-e and g-m, two-tailed Mann-Whitney test, f, one-way ANOVA with Dunn’s multiple comparisons test. Each panel is representative of at least two independent experiments. i, Created with BioRender.com.