Extended Data Fig. 3. Food avoidance in C57BL/6 strain.
a, Cumulative licks of OVA solution from BALB/c or C57BL/6 female mice sensitized with PBS/alum or OVA/alum. After day 1, mice received water bottles, and on two-bottle preference test day 2, OVA bottles were offered on the opposite side of d1 (n = 12 control and 8 allergic BALB/cJ, 17 control and allergic C57BL/6J). b, Preference to OVA solution (n = 14 control and 13 allergic BALB/cJ, 12 control and 20 allergic C57BL/6J) . c, Total serum IgE in mice sensitized with OVA/alum and orally challenged five times (n = 6 control and allergic BALB/cJ, 12 control and 6 allergic C57BL/6J). d, OVA-specific IgG1 after allergic sensitization and challenges (n = 6 control and allergic BALB/cJ, 12 control and 5 allergic C57BL/6J). e, Gastrointestinal transit time was determined on the fourth oral challenge with OVA in control and OVA/alum sensitized mice using a red carmine assay (n = 15 control and 16 allergic BALB/cJ, 6 control and allergic C57BL/6J). f, Systemic corticosterone 1 h after the fifth OVA challenge in control and OVA/alum sensitized BALB/c or C57BL/6 mice (n = 14 control and 13 allergic BALB/cJ, 6 control and allergic C57BL/6J). g, MCPT-1 in the serum 1 h after the fifth oral OVA challenge (n = 6 control and allergic BALB/cJ, 12 control and 9 allergic C57BL/6J). h, Number of chloroacetate esterase (CAE) positive cells present in the jejunum upon 5 oral challenges of control or allergic mice (n = 6 control and 11 allergic BALB/cJ, 6 control and allergic C57BL/6J). i, Representative images of histological sections and CAE staining of OVA/alum-sensitized small intestines (jejunum) from C57BL/6 and BALB/c at magnitude 10x and zoomed images at 40x. Arrows indicate CAE-stained cells. Scale bars = 50 µm. Graphs show mean ± s.e.m. a and c-h, Two-tailed Mann-Whitney test, b, 2-way ANOVA with Turkey’s multiple comparisons test. Each panel is representative of at least two independent experiments.