Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jul 12;620(7974):634–642. doi: 10.1038/s41586-023-06188-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a–c, Egg white water preference of alum-immunized and OVA-alum-immunized BALB/c Igh-7^+/+ and Igh-7^−/− mice displayed as percentage of egg white water intake over total water intake (colour scale indicates percentages) during the course of the IntelliCage experiment (a). Each row corresponds to an individual mouse. The grey field indicates a mouse that had lost the transponder for 1 day, precluding measurement. b, Data from a displayed as egg white water licks over total water licks versus time. Data are presented as mean ± s.e.m. c, Data from a displayed as the fraction of egg white water intake over total water intake as an average per day for the duration of the experiment. d,e, Serum amounts of anti-OVA IgE (d) and anti-OVA IgG1 (e) at the end of the experiment. f,g, BALB/c Cpa3^+/+ and Cpa3^Cre/+ mice received mouse anti-OVA monoclonal IgE antibody on days 0 and 9 and were subjected to the avoidance test (IntelliCage as in Fig. 1b) starting on day 10 (Methods). f, Egg white water preference is displayed as in a. Each row represents an individual mouse. g, Egg white water preference is displayed as in c. h,i, Absolute numbers of stomach mast cells (h) and frequency of small intestine intraepithelial mast cells (i) among total live cells at the end of the experiment. j,k, CD63 expression, an indicator of mast cell activation, on stomach (j) and small intestine intraepithelial (k) mast cells from IgE-sensitized BALB/c Cpa3^+/+ mice after intragastric gavage (i.g.) with OVA or control protein (BSA). l, Rectal temperatures after indicated challenges in IgE-sensitized (passive sensitization) or OVA-immunized (OVA-alum) BALB/c mice. Data are presented as mean ± s.e.m. For a–e, Igh-7^+/+ alum (n = 3 mice); Igh-7^+/+ OVA-alum (n = 7); Igh-7^−/− alum (n = 6); Igh-7^−/− OVA-alum (n = 14). For f–i, Cpa3^+/+ (n = 7); Cpa3^Cre/+ (n = 6). For j–l, Cpa3^+/+ IgE/BSA (n = 6); Cpa3^+/+ IgE/OVA (n = 5); Cpa3^+/+ OVA-alum/OVA (n = 2). Statistical analysis was performed using one-way analysis of variance with Tukey multiple-comparison test (c–e) and two-sided Student’s t tests (g–k). Exact P values are shown. MFI, mean fluorescence intensity.

Source data