Extended Data Fig. 1. Methods for WGS of single cell-derived organoids.
a, Schema of single normal epithelial cell-derived organoid establishment. WGA, whole-genome amplification. b, Methods for mutation calling of WGA samples established via culture method-1 shown in a. c, The number of SNVs (left) and indels (right) in single organoids plotted against the age of participants. Twelve organoids derived from milk of healthy breastfeeding women (healthy volunteers, n = 6) and 20 derived from the normal breast tissue of premenopausal breast cancer patients (n = 6) are shown. Linear regression models assuming a zero intercept were applied to mean number of mutations per participant and age, which are shown in grey dashed lines with R2 and coefficient values, wherein three organoids carrying driver mutations were excluded from the analysis.