Table 1.

Mascott Mass Spectrometry for phosphosites’ identification on AMBRA1 upon Nocodazole treatment

Peptide sequence	Modified amino acid residue	MASCOT score	Asynchronous	Nocodazole
SSASPQEERTV	Ser637	40	–	+
LSSSASPQEERT	Ser639	54	+	+
SQTGTEPGAAHTSSPQPS	Ser1176	37	+	+
LLPEAGQLAERGLSPRT	Ser1198	36	+	–
TSRGLLPEAGQLAERGLSPRTA	Thr1201	26	–	+
SWDQPGTPGREPTQPT	Thr1209	26	–	+
SWDQPGTPGREPTQPTLPSSSPVPIPV	Ser1203, Ser1223	25	–	+

^aThe modified residue is indicated in bold

^dHere we show a selection of sites with a minimum MASCOT score of 25. The complete list of identified sites, with their relative MASCOT score is shown in Dataset 1

^cHere we show sites with AMBRA1 isoform 3 numbering (Uniprot Q9C0C7), as in the whole text