Table 3.
List of predicted proteins in S. sanguinis membrane vesicles that are either predicted as virulence factors in the Virulence Factor Database (VFDB), predicted moonlighting proteins, or predicted as virulence factors in VirPred.
| Ensembl Gene ID | Accession | COG | Number peptides | Number detected | Symbol | Description | VFDB | Moonlighting | VirulentPred |
|---|---|---|---|---|---|---|---|---|---|
| SSA_1882 | A3CQ08 | R | 9 | 4 | prtS | C5a peptidase (prtS) | Yes | Yes | Virulent |
| SSA_0688 | A3CLS0 | G | 2 | 2 | gpmA | 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase | No | Yes | Nonvirulent |
| SSA_0886 | A3CMA7 | G | 2 | 4 | eno | Enolase | Yes | Yes | Nonvirulent |
| SSA_0120 | A3CK77 | J | 2 | 4 | rpsH | 30 S ribosomal protein S8 | No | Yes | Nonvirulent |
| SSA_0176 | A3CKD3 | K | 2 | 4 | rpoB | DNA-directed RNA polymerase subunit beta | No | Yes | Nonvirulent |
| SSA_0068 | A3CK27 | C | 1 | 1 | adhE | Aldehyde-alcohol dehydrogenase | No | Yes | Nonvirulent |
| SSA_1949 | A3CQ69 | E | 1 | 1 | SSA_1949 | AliA protein | No | Yes | Nonvirulent |
| SSA_0302 | A3CKQ4 | G | 1 | 1 | pgk | Phosphoglycerate kinase | No | Yes | Nonvirulent |
| SSA_0908 | A3CMC7 | R | 1 | 1 | SSA_0908 | ABC-type uncharacterized transport system, periplasmic component, | No | Yes | Nonvirulent |
| SSA_0804 | A3CM30 | G | 1 | 2 | glmM | Phosphoglucosamine mutase | No | Yes | Nonvirulent |
| SSA_0848 | A3CM70 | G | 1 | 2 | pykF | Pyruvate kinase | No | Yes | Nonvirulent |
| SSA_0263 | A3CKL7 | O | 1 | 2 | pepO | Zinc metalloproteinase in scaA 5’region | No | Yes | Nonvirulent |
| SSA_0721 | A3CLV0 | P | 1 | 2 | sodA | Superoxide dismutase | No | Yes | Nonvirulent |
| SSA_0453 | A3CL46 | E | 1 | 3 | SSA_0453 | Alpha-dextrin endo-1,6-alpha-glucosidase | No | Yes | Nonvirulent |
| SSA_0847 | A3CM69 | G | 1 | 3 | pfk | ATP-dependent 6-phosphofructokinase | No | Yes | Nonvirulent |
| SSA_0860 | A3CM81 | M | 1 | 3 | SSA_0860 | Lysozyme, GN = SSA_0860 | No | Yes | Virulent |
| SSA_0015 | A3CJX9 | O | 1 | 3 | ftsH | ATP-dependent zinc metalloprotease | No | Yes | Nonvirulent |
| SSA_1992 | A3CQA8 | G | 1 | 4 | fba | Fructose-bisphosphate aldolase | Yes | Yes | Nonvirulent |
| SSA_1283 | A3CNC9 | C | 1 | 4 | nrd | Nitroreductase | No | Yes | Nonvirulent |
| SSA_1732 | A3CPK9 | F | 1 | 4 | upp | Uracil phosphoribosyltransferase | No | Yes | Nonvirulent |
| SSA_2109 | A3CQM2 | J | 1 | 4 | fusA | Elongation factor G | No | Yes | Nonvirulent |
| SSA_1817 | A3CPU4 | R | 1 | 4 | SSA_1817 | Conserved uncharacterized protein | No | Yes | Virulent |
| SSA_1099 | A3CMV6 | Q | 4 | 4 | SSA_1099 | Calcium binding hemolysin-like protein, GN = SSA_1099 | No | No | Virulent |
| SSA_0613 | A3CLJ9 | R | 3 | 4 | gtfP | Dextransucrase, glucosyltransferase gtfP | Yes | No | Nonvirulent |
| SSA_1797 | A3CPS4 | R | 2 | 4 | pabC | Endolytic murein transglycosylase | No | No | Virulent |
| SSA_1245 | A3CN93 | K | 1 | 1 | cpsY | Transcriptional regulator, LysR family | No | No | Virulent |
| SSA_1249 | A3CN97 | L | 1 | 1 | SSA_1249 | CRISPR system Cms endoribonuclease Csm3 | No | No | Virulent |
| SSA_2278 | A3CR35 | S | 1 | 1 | ukp | Ukp protein | No | No | Virulent |
| SSA_1505 | A3CNZ4 | M | 1 | 2 | SSA_1505 | Sulfatase domain-containing protein | No | No | Virulent |
| SSA_2004 | A3CQB9 | O | 1 | 3 | zmpB | Zinc metalloprotease zmpB | Yes | No | Virulent |
| SSA_1854 | A3CPY0 | D | 1 | 3 | SSA_1854 | Mid-cell-anchored protein Z | No | No | Virulent |
| SSA_1832 | A3CPV9 | J | 1 | 3 | SSA_1832 | UPF0374 protein | No | No | Virulent |
| SSA_0159 | A3CKB5 | S | 1 | 3 | SSA_0159 | Uncharacterized protein | No | No | Virulent |
| SSA_1909 | A3CQ29 | K | 1 | 4 | SSA_1909 | Transcriptional attenuator LytR | No | No | Virulent |
| SSA_0647 | A3CLN0 | P | 1 | 4 | SSA_0647 | Rhodanese domain-containing protein | No | No | Virulent |
| SSA_1291 | A3CND6 | V | 1 | 4 | SSA_1291 | Tox-GHH domain-containing protein | No | No | Virulent |
COG refers to the cluster of orthologous groups for each protein, Number peptides is the number of significant peptides of each predicted protein. Number detection is the number of independent vesicle preparations each predicted protein is found, in a total of 4 preparations.