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. 2023 Jul 28;9(8):e18789. doi: 10.1016/j.heliyon.2023.e18789

Fig. 3.

Fig. 3

ZFP36L2 was a direct target gene of miR-520d-3p. (A) Complementarity of the 3′-UTR of wild-type (WT) or mutant (MUT) human ZFP36L2 mRNA with the miR-520d-3p seed sequence. (B, C) Relative luciferase activity in HeLa and SiHa cell line co-transfected with NC mimics/miR-520d-3p mimics and ZFP36L2-WT/MUT plasmid. (D) Reverse transcription quantitative PCR analyses of ZFP36L2 mRNA expression in HeLa and SiHa cells following transfection of miR-520d-3p mimics or NC mimics. The values presented as mean ± SD (n = 3). *p < 0.05, **p < 0.01, compared with NC mimics. (E) Western blotting results of ZFP36L2 expression following transfection of NC mimics and miR-520d-3p mimics into HeLa and SiHa cells.