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. 2023 Jul 28;9(8):e18789. doi: 10.1016/j.heliyon.2023.e18789

Fig. 5.

Fig. 5

Overexpression of ZFP36L2 rescued the effects of miR-520d-3p on CC cell proliferation and migration. HeLa and SiHa cells were transfected with pcDNA-ZFP36L2 and the miR-520d-3p mimics individually or in combination. (A) The overexpression of ZFP36L2 was confirmed by Western blot analysis in HeLa and SiHa cells transfected with pcDNA-ZFP36L2 individually. (B) ZFP36L2 overexpression reversed the suppressive effect of miR-520d-3p mimics on ZFP36L2 expression. (C, D) Cell proliferation was determined by CCK-8 assay in transfected HeLa and SiHa cells. (E, F) A wound healing assay was utilized to evaluate the migration of transfected HeLa and SiHa cells. The values presented as mean ± SD (n = 3). **p < 0.01, ***p < 0.001, compared with NC mimics + pcDNA; ###p < 0.001, compared with miR-520d-3p mimics + pcDNA; (G) The protein levels of E-cadherin, N-cadherin and Vimentin were detected by Western blot analysis in transfected HeLa and SiHa cells.