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. 2023 Aug 16;11(8):e007031. doi: 10.1136/jitc-2023-007031

Figure 4.

Figure 4

15(S)-HETE treatment favors the presence of immunocompetent LPM. (A) SPM and LPM were isolated from the ascites of untreated or 15(S)-HETE-treated mice at day 18 post-ID8 injection using F4/80 and MHCII markers. Their respective phenotypes were determined by gene expression analysis of chemotaxis, immunosuppression, angiogenesis, metastasis factors and of proinflammatory or anti-inflammatory cytokines using RT-qPCR. (B) Protein levels of chemotactic factors and proinflammatory or anti-inflammatory cytokines in tumor ascites at days 7, 18 and 35 post-tumor cell injection were evaluated by flow cytometry. Results correspond to mean±SEM (n=6 per group) and are representative of at least three independent experiments. *p<0.05, **p<0.01 and ***p<0.001 compared with respective untreated ID8 tumor-bearing mice. #p<0.05, ##p<0.01, ###p<0.001 and ####p<0.0001 compared with SPM from untreated ID8 tumor-bearing mice. HETE, hydroxyeicosatetraenoic acid; IL, interleukin; LPM, large peritoneal macrophages; mRNA, messenger RNA; RT-qPCR, real-time quantitative PCR; SPM, small peritoneal macrophages; TNF, tumor necrosis factor.