Skip to main content
. 2023 Aug 16;11(8):e007199. doi: 10.1136/jitc-2023-007199

Figure 1.

Figure 1

Cell surface expression of EDB-targeting chimeric receptors and incorporation into the TCR complex. (A) Schematic representation of chimeric antigen receptors containing L19 scFv. BBz and 28z: second-generation CARs; EFL, EFL28, EFL137: rTCR-CARs. (B) Immunoprecipitation and Western blot analysis of second-generation CARs or rTCR-CAR complexes using transduced and purified EDB CAR+ Jurkat T cells. TCRɑ, TCRβ, CD3ζ, CD3γ, and CD3δ are associated with rTCR-CARs, but not second-generation CARs. (C) rTCR-CARs but not second-generation CARs can be precipitated by using anti-TCRβ antibodies. (D) Whole-cell lysate Western blotting using anti-CD3ε antibody; (E) CD3ζ KO Jurkat T cells on transduction expressed second-generation CAR but not rTCR-CAR on the surface of cell membranes. (F) Schematics of the predicted rTCR-CAR structures and their differences from second-generation CARs. co-IP; coimmunoprecipitation; EDB, extra domain B; scFv, single-chain variable fragment.