Figure 5.

Infiltration and persistence of EFL28 rTCR-CAR T cells in large tumors in vivo. (A) 10⁷ CAR T cells or T cells were injected into the tail vein of U87MG tumor-bearing mice, and tumor tissues were collected on the 14th day. Infiltrating T cells were detected by immunohistochemistry using an anti-CD3 antibody. Red arrow heads denote CD3⁺ CAR T cells (B) Quantification of CD3⁺ cells in tumor tissues. Statistical significance was calculated by one-way analysis of variance (ANOVA) with Bonferroni post hoc test, n=5, **p<0.01. (C) 10⁷ CAR T cells were injected into the tail vein of U87MG tumor-bearing mice, and peripheral blood was collected on days 0, 1, 7, 14, 21, and 28 to extract DNA. The copy number of the CAR gene or T cells in peripheral blood was detected by qPCR, n=5. Statistical significance was calculated by one-way ANOVA with Bonferroni posttest, ***p<0.001; ^###p<0.001.