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. 2023 Aug 16;11(8):e007199. doi: 10.1136/jitc-2023-007199

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© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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EFL28 receptor-mediated T-cell activation signaling pathway. (A) The effector cells were incubated with 5 µg/mL EDB protein for the indicated times. The phosphorylation levels of CD3ζ, ERK, and ZAP70 and (B) the protein levels of p65 and NFATc1 in whole-cell lysates were detected by Western blotting. (C) Jurkat^{LCK KO} or (D) Jurkat^{LAT KO} cells and wild-type Jurkat cells as controls were transduced with lentivirus to n for the indicated times. The phosphorylation levels of CD3ζ and ERK in whole-cell lysates were detected by Western blotting. EDB, extra domain B.