TABLE 1.

Synthetic peptides derived from the HSRV Gag sequence and analyzed for susceptibility to cleavage by the HSRV PR

No. of peptide	Peptide sequencea	Position within Gag	PR assay result	Turnover (nmol/60 min [%])
1	PRAVNPHTMFMIS	76–87	Not cleaved
2	DELEDVLNTQSEI	141–153	Not cleaved
3	RSFSGLPSLPSIPGRb	199–211	Not cleaved
4	PIQHIR↓SVTGE	306–316	Cleaved	0.25 (15)
5	PAIDGVF↓PVTTPDLRCRIIN↓AILGGNI	333–359	Cleaved twice	NDc
6	PDGVF↓PVTTPDLRb	336–347	Cleaved	ND
7	PRCRIIN↓AILGG	347–357	Cleaved	0.60 (36)
8	PGNIGLSLTPGDCLTWDS	357–372	Not cleaved
9	PMHQLGNVIKG	387–397	Not cleaved
10	PRAVN↓TVTQRb	618–625	Cleaved	1.65 (100)

^aN termini were modified by adding a Pro residue for fluorogenic assays. Residues in boldface flank the cleavage sites (arrows). 

^bC termini were modified by adding an Arg residue for higher solubility. 

^cND, not done.