Blocking solution
| Reagent | Final concentration | Amount |
|---|---|---|
| Donkey serum | 10% | 500 μL |
| Triton X-100 | 0.3% | 15 μL |
| PBS 0.1 M | 4485 μL | |
| Total | 5 mL |
Store at +4° for all the duration of the protocol.
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