REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-LC3	Abcam	Cat#ab192890; RRID: AB_2827794
Anti-BECLIN1	Abcam	Cat#ab207612; RRID: AB_2692326
Anti-p62	Abcam	Cat#ab109012; RRID: AB_2810880
Anti-RNF125	Abclonal	Cat#A15165; RRID: AB_2762054
Anti-HA (rabbit host)	Cell Signaling Technology	Cat#3724; RRID: AB_1549585
Anti-Ubiquitin	Cell Signaling Technology	Cat#58395
Anti-HA (mouse host)	GIN (Japan)	Cat#GNI4110-HA
Anti-Myc (mouse host)	GIN (Japan)	Cat#GNI4110-MC
Anti-HMGB1 (mouse host)	Novus Biologicals	Cat#NBP2-27396
Anti-HMGB1 (rabbit host)	Proteintech Group	Cat#10829-1-AP; RRID: AB_2232989
Anti-Flag (rabbit host)	Proteintech Group	Cat#20543-1-AP; RRID: AB_11232216
Anti-Myc (rabbit host)	Proteintech Group	Cat#16286-1-AP; RRID: AB_11182162
Anti-β-actin	Proteintech Group	Cat#20536-1-AP; RRID: AB_10700003
Anti-ZO-1	Proteintech Group	Cat#21773-1-AP; RRID: AB_10733242
Anti-OCCLUDIN	Proteintech Group	Cat#13409-1-AP; RRID: AB_2156308
Anti-CLAUDIN 1	Proteintech Group	Cat#13050-1-AP; RRID: AB_2079881
Anti-Flag (mouse host)	Sigma-Aldrich	Cat#F1804; RRID: AB_262044
Chemicals, peptides, and recombinant proteins
LPS	Sigma-Aldrich	Cat#L2880
Ovalbumin	Sigma-Aldrich	Cat#A5503
DCFH-DA	Sigma-Aldrich	Cat#D6883
AL(OH)3	Thermo Scientific	Cat#77161
RPMI-1640 medium	Procell	Cat#PM150110
Fetal bovine serum	Bioind	Cat#04-001
Pronase	Solarbio	Cat#P8360
DNAseI	Solarbio	Cat#D8071
TRIzol Reagent	Takara	Cat#9108
Phosphatase inhibitor	Meilunbio	Cat#MB12707
Phenylmethanesulfonyl fluoride	Solarbio	Cat#IP0280
Decitabine	Selleckchem	Cat#S1200
MG132	ApexBio	Cat#a2585
CHX	Aladdin	Cat#C112766
Critical commercial assays
Wright–Giemsa stain kit	Solarbio	Cat#G1020
Periodic acid-Schiff kit	Beyotime	Cat#C0142
Malondialdehyde kit	Beyotime	Cat#S0131
superoxide dismutase kit	Elabscinece	Cat#E-BC-K020
Glutathione kit	Solarbio	Cat#BC1175
BCA kit	Beyotime	Cat#P0010
cDNA Synthesis Kit	Takara	Cat#RR047A
SYBR PrimeScript RT-PCR Kit	Takara	Cat#RR820A
Genomic DNA Extraction Kit	Sangon Biotech	Cat#B518264
Deposited data
Mass data (RNF125 interacting protein)	Beijing Protein Innovation	ProteomeXchange: PXD043937; iProX: IPX0006363000
Mass data (HMGB1 ubiquitination related sites)	Shanghai Applied Protein Technology	ProteomeXchange: PXD043938; iProX: IPX0006367000
Experimental models: Cell lines
Human bronchial epithelial cell line 16HBE	Xiangya School of Medicine (Changsha, China)	Cat#HTCC23
Experimental models: Organisms/strains
Female BALB/c mice (6–7 weeks old, 17–18 g)	Beijing Hfk Bioscience Co. Ltd.	Cat#11002A
Oligonucleotides
Primer:human RNF125 Forward	This paper	5ʹ-GTTCCTGTATTGCTACCAGTCT-3ʹ
Primer:human RNF125 Reverse	This paper	5ʹ-TTCTTTTGGCTACATCAGTTGC-3ʹ
Primer:human HMGB1 Forward	This paper	5ʹ-TCTTCCTCTTCTGCTCTGAGTATCG-3ʹ
Primer:human HMGB1 Reverse	This paper	5ʹ-TTCGCAACATCACCAATGGACAG-3ʹ
Primer:human β-actin Forward	This paper	5ʹ-CCTGGCACCCAGCACAAT-3ʹ
Primer:human β-actin Reverse	This paper	5ʹ-GGGCCGGACTCGTCATAC-3ʹ
Recombinant DNA
Flag-RNF125	Han bio	N/A
Myc-HMGB1 wt	Sino Biological	N/A
Myc-HMGB1 (1-163aa)	Sino Biological	N/A
Myc-HMGB1 (1-79aa)	Sino Biological	N/A
Myc-HMGB1 (80-215aa)	Sino Biological	N/A
Software and algorithms
R 3.6.3	The R Foundation	https://www.r-project.org/
UCSC	Navarro Gonzalez59	http://genome.ucsc.edu
Gene Ontology	Ashburner et al.60	http://geneontology.org
ClusterProfiler	Yu et al.61	http://bioconductor.org/packages/release/bioc/html/clusterProfiler.html
org.Hs.e.g.,.db	Carlson M et al.62	http://bioconductor.org/packages/release/data/annotation/html/org.Hs.eg.db.html
ImageJ	National Institutes of Health	https://imagej.net/Fiji
GraphPad Prism 8.0	GraphPad	https://www.graphpad.com/