Fig. 6.

Zinc sulfate-containing FMD vaccine effectively elicit cellular and humoral immune responses in porcine PBMCs by inducing the expression of PRRs, transcription factors, co-stimulatory molecules, and cytokines. (A–V) Gene expression levels of RIG-I (A); TLR4 (B); MyD88 (C); TRAF6 (D); NF-κB (E); CARD9 (F); STAT1 (G); CD80 (H); CD86 (I); CD28 (J); CD19 (K); CD21 (L); CD81 (M); IFNα (N); IFNβ (O); IFNγ (P); IL-1β (Q); IL-6 (R); IL-12p40 (S); IL-23p19 (T); IL-23R (U); and IL-17A (V). ^⁎p < 0.05, ^⁎⁎p < 0.01, ^⁎⁎⁎p < 0.001, and ^⁎⁎⁎⁎p < 0.0001.