Table 1.
The composition of experimental diets.1
| Item | Phase 1 |
Phase 2 |
Phase 3 |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NC | PC | RFM | RPM | RBP | NC | PC | RFM | RPM | RBP | NC | PC | RFM | RPM | RBP | |
| Feedstuff, % as-is basis | |||||||||||||||
| Corn, yellow | 28.99 | 26.68 | 27.65 | 31.62 | 25.61 | 40.67 | 40.10 | 39.89 | 42.59 | 38.92 | 62.57 | 62.31 | 62.22 | 63.52 | 61.66 |
| Whey permeate | 24.00 | 24.00 | 24.00 | 24.00 | 24.00 | 15.00 | 15.00 | 15.00 | 15.00 | 15.00 | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 |
| Soybean meal, 48% CP | 16.00 | 16.00 | 16.00 | 16.00 | 16.00 | 19.00 | 19.00 | 19.00 | 19.00 | 19.00 | 23.00 | 23.00 | 23.00 | 23.00 | 23.00 |
| Cookie meal | 10.00 | 10.00 | 10.00 | 10.00 | 10.00 | 10.00 | 10.00 | 10.00 | 10.00 | 10.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| Poultry meal | 10.00 | 0.00 | 10.00 | 0.00 | 10.00 | 8.00 | 0.00 | 8.00 | 0.00 | 8.00 | 4.00 | 0.00 | 4.00 | 0.00 | 4.00 |
| Fish meal | 4.00 | 0.00 | 0.00 | 4.00 | 4.00 | 2.00 | 0.00 | 0.00 | 2.00 | 2.00 | 1.00 | 0.00 | 0.00 | 1.00 | 1.00 |
| Blood plasma | 4.00 | 0.00 | 4.00 | 4.00 | 0.00 | 2.00 | 0.00 | 2.00 | 2.00 | 0.00 | 1.00 | 0.00 | 1.00 | 1.00 | 0.00 |
| SPC2 | 0.00 | 18.50 | 5.00 | 6.00 | 7.50 | 0.00 | 11.05 | 2.50 | 4.80 | 3.75 | 0.00 | 5.55 | 1.25 | 2.40 | 1.90 |
| Poultry fat | 1.00 | 1.70 | 1.00 | 1.70 | 1.00 | 1.00 | 1.60 | 1.00 | 1.60 | 1.00 | 1.00 | 1.30 | 1.00 | 1.30 | 1.00 |
| L-Lys HCl | 0.52 | 0.55 | 0.53 | 0.52 | 0.54 | 0.51 | 0.53 | 0.51 | 0.51 | 0.52 | 0.42 | 0.42 | 0.42 | 0.42 | 0.42 |
| L-Met | 0.25 | 0.24 | 0.25 | 0.22 | 0.26 | 0.21 | 0.20 | 0.21 | 0.19 | 0.22 | 0.14 | 0.14 | 0.14 | 0.14 | 0.15 |
| L-Thr | 0.17 | 0.03 | 0.12 | 0.13 | 0.12 | 0.15 | 0.08 | 0.13 | 0.12 | 0.13 | 0.12 | 0.08 | 0.12 | 0.12 | 0.12 |
| L-Trp | 0.02 | 0.00 | 0.00 | 0.01 | 0.02 | 0.01 | 0.00 | 0.01 | 0.00 | 0.01 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| L-Val | 0.00 | 0.00 | 0.00 | 0.05 | 0.00 | 0.00 | 0.01 | 0.00 | 0.04 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| Limestone, ground | 0.40 | 0.80 | 0.60 | 0.85 | 0.30 | 0.60 | 0.83 | 0.70 | 0.85 | 0.60 | 0.80 | 0.90 | 0.80 | 0.80 | 0.80 |
| Dicalcium phosphate | 0.00 | 0.85 | 0.20 | 0.25 | 0.00 | 0.20 | 0.95 | 0.40 | 0.65 | 0.20 | 0.55 | 0.90 | 0.65 | 0.90 | 0.55 |
| Zinc oxide3 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| Salt | 0.22 | 0.22 | 0.22 | 0.22 | 0.22 | 0.22 | 0.22 | 0.22 | 0.22 | 0.22 | 0.22 | 0.22 | 0.22 | 0.22 | 0.22 |
| Mineral premix4 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 |
| Vitamin premix5 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 |
| Calculated composition, % as-is basis | |||||||||||||||
| DM | 91.2 | 91.6 | 91.4 | 91.1 | 91.6 | 90.7 | 90.8 | 90.8 | 90.5 | 90.8 | 89.7 | 89.8 | 89.7 | 89.6 | 89.8 |
| ME, kcal/kg | 3,436 | 3,431 | 3,434 | 3,438 | 3,440 | 3,419 | 3,414 | 3,415 | 3,418 | 3,420 | 3,372 | 3,372 | 3,372 | 3,372 | 3,373 |
| Crude protein | 24.6 | 23.6 | 25.0 | 22.0 | 25.8 | 22.6 | 21.3 | 22.8 | 20.5 | 23.2 | 20.9 | 20.2 | 21.0 | 19.8 | 21.2 |
| SID6 Lys | 1.50 | 1.50 | 1.50 | 1.50 | 1.50 | 1.35 | 1.35 | 1.35 | 1.35 | 1.35 | 1.23 | 1.23 | 1.23 | 1.23 | 1.23 |
| SID Met + Cys | 0.82 | 0.82 | 0.82 | 0.82 | 0.82 | 0.74 | 0.74 | 0.74 | 0.74 | 0.74 | 0.68 | 0.68 | 0.68 | 0.69 | 0.68 |
| SID Trp | 0.25 | 0.26 | 0.25 | 0.25 | 0.25 | 0.22 | 0.23 | 0.23 | 0.22 | 0.22 | 0.20 | 0.21 | 0.21 | 0.20 | 0.20 |
| SID Thr | 0.88 | 0.88 | 0.88 | 0.88 | 0.88 | 0.79 | 0.79 | 0.79 | 0.79 | 0.79 | 0.74 | 0.73 | 0.74 | 0.74 | 0.74 |
| Ca | 0.88 | 0.85 | 0.85 | 0.85 | 0.87 | 0.80 | 0.80 | 0.81 | 0.80 | 0.81 | 0.71 | 0.70 | 0.70 | 0.70 | 0.71 |
| STTD P7 | 0.50 | 0.45 | 0.46 | 0.45 | 0.48 | 0.41 | 0.40 | 0.40 | 0.40 | 0.40 | 0.33 | 0.33 | 0.33 | 0.33 | 0.33 |
| Total P | 0.73 | 0.67 | 0.69 | 0.64 | 0.73 | 0.64 | 0.63 | 0.64 | 0.61 | 0.64 | 0.58 | 0.57 | 0.58 | 0.57 | 0.58 |
| Glycinin,8 mg/kg | 18.0 | 18.0 | 18.0 | 18.0 | 18.0 | 21.4 | 21.4 | 21.4 | 21.4 | 21.4 | 25.9 | 25.9 | 25.9 | 25.9 | 25.9 |
| β-Conglycinin,9 mg/kg | 20.0 | 20.0 | 20.0 | 20.0 | 20.0 | 23.8 | 23.8 | 23.8 | 23.8 | 23.8 | 28.8 | 28.8 | 28.8 | 28.8 | 28.8 |
| Analyzed composition, % as-is basis | |||||||||||||||
| DM | 91.8 | 91.7 | 91.4 | 91.0 | 91.8 | 90.3 | 90.5 | 90.3 | 90.0 | 90.9 | 88.9 | 88.8 | 88.8 | 88.2 | 89.0 |
| Crude ash | 6.76 | 6.75 | 6.56 | 6.59 | 6.60 | 6.25 | 6.37 | 6.22 | 6.19 | 6.54 | 4.74 | 4.88 | 4.37 | 4.66 | 4.88 |
| CP | 24.7 | 22.5 | 24.3 | 21.1 | 25.3 | 22.1 | 20.7 | 22.8 | 19.7 | 23.1 | 20.7 | 19.7 | 19.1 | 18.9 | 20.8 |
| Neutral detergent fiber | 6.80 | 6.08 | 7.09 | 5.42 | 7.57 | 7.36 | 6.64 | 7.82 | 6.82 | 7.88 | 6.42 | 7.09 | 7.21 | 7.14 | 7.90 |
| Acid detergent fiber | 3.09 | 3.48 | 3.2 | 2.89 | 3.45 | 3.58 | 3.87 | 3.63 | 3.26 | 3.69 | 2.94 | 3.22 | 2.85 | 3.07 | 3.46 |
| Ca | 0.91 | 0.78 | 0.75 | 0.81 | 0.79 | 0.80 | 0.83 | 0.77 | 0.81 | 0.89 | 0.76 | 0.73 | 0.76 | 0.77 | 0.82 |
| Total P | 0.77 | 0.65 | 0.68 | 0.63 | 0.71 | 0.67 | 0.66 | 0.69 | 0.66 | 0.69 | 0.55 | 0.55 | 0.48 | 0.56 | 0.58 |
SPC = soy protein concentrate; DM = dry matter.
NC, PC, RFM, RPM, and RBP: basal diet without SPC; basal diet with SPC replacing all animal protein supplements; basal diet with SPC replacing fish meal; basal diet with SPC replacing poultry meal; basal diet with SPC replacing blood plasma.
SPC (X-Soy 200, Selecta, MG, Brazil).
Zinc oxide was targeted to 2,500 mg/kg in phase 1 and phase 2 diets.
The trace mineral premix provided per kilogram of complete diet: 33 mg of Mn as manganous oxide, 110 mg of Fe as ferrous sulfate, 110 mg of Zn as zinc sulfate, 16.5 mg of Cu as copper sulfate, 0.30 mg of I as ethylenediamine dihydroiodide, and 0.30 mg of Se as sodium selenite.
The vitamin premix provided per kilogram of complete diet: 6,614 IU of vitamin A as vitamin A acetate, 992 IU of vitamin D3, 19.8 IU of vitamin E, 2.64 mg of vitamin K as menadione sodium bisulfate, 0.03 mg of vitamin B12, 4.63 mg of riboflavin, 18.52 mg of D-pantothenic acid as calcium pantothenate, 24.96 mg of niacin, and 0.07 mg of biotin.
SID, standardized ileal digestibility.
STTD P, standardized total tract digestible phosphorus.
The concentration of glycinin in the diets was calculated based on the analyzed glycinin of soybean meal and soy protein concentrate.
The concentration of β-conglycinin in the diets was calculated based on the analyzed β-conglycinin of soybean meal and soy protein concentrate.