Fig. 4.

Glycosidase shear-sensitivity analyses of CA IX WT and its mutant forms. A) Western blot analysis of purified proteins CA IX WT, MUT1 and MUT4 digested and non-digested with EndoH. All digested samples present an electrophoretic shift when compared to undigested controls. B) Western blot analysis of purified proteins CA IX WT and mutants digested with O-glycosidase alone and combined with Neuraminidase. Undigested samples are used as negative control. Only CA IX WT and MUT4 show an electrophoretic shift. Uncropped images of the Western blot analyses are available as supplementary material (Succoio_supplementary_materials).