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Brefeldin A impairs nuclear translocation of the wild-type CA IX protein. Immunolocalization of golgin (green) with CA IX (red) in SH-SY5Y cells with and without BFA treatment. In CA IX WT, after BFA treatment, cells show a significant increase in CA IX the perinuclear signal and in vesicle quantity, whereas MUT4 shows mostly perinuclear signal. Bars: on left panels = 5 μm, on right panels = 3 μm.
