A. QPLI microscope images depicting fiber orientation in scaffolds after 1 day of culture, red is vertical fibers and cyan is horizontal. Scale bar = 5 mm. B. Confocal reflectance images visualizing collagen I fibers in acellular hydrogels after 2 days of culture. Scale bar = 100 μm. C. Graph showing the directional variance of samples with and without stretching. Analysis performed with multiple t-tests, *p<0.05 for n = 4/group. D. Graph showing the coherence (uniformity) for each sample group. Analysis performed with multiple t-tests, *p<0.05, ****p<0.001 for n = 12/group. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)