Fig. 3.

A. Images of cells in the bulk of the hydrogel after 1 week of culture, stained with phalloidin (red) to visualize f-actin and the cell body and with DAPI (blue) to visualize cell nuclei. Scale bar = 100 μm B, C. Graph depicting the coherence (alignment) of actin fibers within (B) 3 mg/mL and (C) 6 mg/mL samples. D. Graph showing the coherence of actin fibers within stretched scaffolds for 3 and 6 mg/mL and 1 and 2 M/mL. Analysis performed with multiple t-tests. ****p<0.0001 for n = 12/group. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)