Fig. 9.

A. Immunofluorescent images of neurite outgrowth after 48 h of co-culture on 3 mg/mL scaffolds visualizing neurofilament (red) and f-actin within cells (green). Scale bar = 500 μm B. Neurite orientation in 3 mg/mL 1 M/mL scaffolds, with values binned every 10°. C, D, E. Neurite count over a certain distance: (C) 100–1400 μm, (D) 500 μm, (E) 1400 μm. Analysis for C performed with three-way ANOVA. Analysis for D and E performed with two-way ANOVA and post-hoc Tukey's test. *p<0.05 for n = 4/group. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)