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. 2023 Jul 17;169(7):001364. doi: 10.1099/mic.0.001364

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© 2023 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.

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Fig. 2. — IS subgroups generally show host lineage-specificity and widespread IS are found in different genomic elements depending on host phylotype. (a) Heatmap of IS subgroup prevalence across the RSSC phylogeny separated by genomic element (chromosome on the left and megaplasmid on the right). IS subgroups are clustered by IS family and shown with coloured bars above the heatmaps. Scale bar (nucleotide substitutions per site) is shown below. (b) PCoA plot based on pairwise isolate IS content Bray-Curtis dissimilarities. Points are coloured by host phylotypes and ellipses show predicted phylotype distributions around the centroid. Phylotype IIB does not show an ellipse due to bimodal distribution caused by clonal and non-clonal sub-lineages. Top-left shows the results of ANOSIM including the R statistic (measure of phylotype IS distinguishability), P-value, and number of permutations. (c) Tanglegram representing the analysis of congruence between phylotype I isolate phylogeny (left side) and UPGMA tree (right side) calculated using Bray-Curtis dissimilarity of IS presence.