Table 4.
Carbon phenotypes of putative fructose transport mutants
|
Strain |
Relevant genotype Chromosomal (plasmid) |
LB |
Sbt |
Mtl |
Fru |
Glu |
|---|---|---|---|---|---|---|
|
Rm1021 |
wt |
+ |
+ |
+ |
+ |
+ |
|
SRmD495 |
smoK::Tn5-B20 |
+ |
+/− |
+/− |
+ |
+ |
|
SRmD664 |
frcA::pKNOCK-Gm |
+ |
+/− |
+/− |
+/− |
+ |
|
SRmD665 |
frcC::pKNOCK-Gm |
+ |
+/− |
+/− |
+/− |
+ |
|
SRmD666 |
smoK, frcA |
+ |
− |
− |
− |
+ |
|
SRmD666 (SMc01500) |
smoK, frcA (smoS) |
+ |
− |
− |
− |
+ |
|
SRmD666 (pJD02) |
smoK, frcA (mtlK) |
+ |
− |
− |
− |
+ |
|
SRmD667 |
smoK, frcC |
+ |
− |
− |
− |
+ |
|
SRmD667 (SMc01500) |
smoK, frcC (smoS) |
+ |
− |
− |
− |
+ |
|
SRmD667 (pJD02) |
smoK, frcC (mtlK) |
+ |
− |
− |
− |
+ |
Strains were streaked onto complex (LB) or defined (VMM) agar medium containing caron sources as indicated in the table. Growth scored as follows; +, like wild-type; -, no growth; +/−, intermediate growth. Abbreviations are as follows: LB, Luria–Bertani; sbt, sorbitol; mtl, mannitol; fru, fructose; glu, glucose.