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. 2023 Aug 3;50(3):172. doi: 10.3892/or.2023.8609

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Differential expression of SPAG5 in hepatoma cells and normal hepatocytes and tissues. (A) The differential expression of SPAG5 in MIHA and Huh7 observed by fluorescence microscopy. The blue signal represents the nuclear DNA staining of DAPI, and the green signal represents the staining of SPAG5. (B and C) Immunohistochemical staining revealed SPAG5 expression in 15 patients. (D) Reverse transcription-quantitative PCR analysis of the relative mRNA levels of SPAG5 in MIHA, Huh7 and HCCLM3 cell lines. (E and F) Western blot analysis of SPAG5 expression in MIHA, Huh7 and HCCLM3 cell lines. **P<0.01, ***P<0.001 and ****P<0.0001. SPAG5, sperm-associated antigen 5.