Fig. 3.

Ca²⁺ spark events are comparable between normotensive and hypertensive vessels. (A) Cerebral pial arteries from normotensive and hypertensive mice loaded with Fluo-4-AM, pressurized to 60 mmHg, and imaged using a spinning-disk confocal microscope. (A, ii) ST maps generated from recordings processed using Volumetry. Events were converted to pixels and given a ZScr to allow categorization into sparks and waves based on duration (length) and spatial spread (width). Summary data showing spark frequency, amplitude, duration, and spread (B) in normotensive (gray) and hypertensive (green) mice (n = 7 arteries from 7 normotensive mice and 10 arteries from 10 hypertensive mice; unpaired t test).