Fig. 2.

Determination of myonuclei originated from NG2⁺ PCs. A Myofibers isolated from the soleus, measured area of each myofiber, and the number of myonuclei. B The calculated ratio of the myonuclear number to that of myofibers. The values are presented as mean ± SEM (n = 3). ns = not significant. C. NG2⁺ PC-originated nuclei within myofibers, determined by fluorescence in situ hybridization (FISH). A non-specific DNA probe was used as a negative control. Nuclei are stained by Hoechst 33,342. Scale bar = 50 µm