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. 2023 Jul 25;26(3):391. doi: 10.3892/ol.2023.13977

Figure 3.

Figure 3.

GRHL2 silencing induces a partial EMT in hTERT/Cdk4-immortalized normal lung epithelial cell line (HBEC4KT) without affecting its growth. (A) Western blotting of GRHL2, E-cadherin and Vimentin in the immortalized normal bronchial epithelial cell line HBEC4KT transfected with either control or GRHL2 siRNA. Values below bands indicate quantitation of band intensities normalized to β-actin. Quantification of band intensities is shown in the right graph. (B) Reverse transcription-quantitative PCR of E-cadherin, VIMENTIN and four master EMT regulators in HBEC4KT cells, transfected with either control (si-NC) or GRHL2 siRNA. (C) Proliferation and (D) colony formation assays for HBEC4KT cells transfected with either control or GRHL2 siRNA. Results are shown as the mean ± SD (n=3), where comparisons were performed with one-way ANOVA followed by the Dunnett test. **P<0.01 and ***P<0.001 vs. si-NC. GRHL2, Grainyhead-like 2, siRNA, small-interfering RNA; NC, negative control; n.s., non-significant; EMT, epithelial-to-mesenchymal transition; ZEB, Zinc finger E-box-binding homeobox; SNAI, snail homolog.