TABLE 1.
Infection of cells expressing the R-receptor by RD114, BaEV, and type D simian retroviruses
| Expt | Target cellsb | Titer of lacZ pseudotype (CFU/ml)a
|
||||
|---|---|---|---|---|---|---|
| RD114 | BaEV | MPMV | SRV-1 | SRV-2 | ||
| 1 | TE671 | 2.1 × 106 | — | — | — | — |
| NIH 3T3 | 7 | — | — | — | — | |
| 3T3R16 | 4.7 × 105 | — | — | — | — | |
| CHO | 0 | — | — | — | — | |
| CHOR16 | 1.5 × 105 | — | — | — | — | |
| 2 | TE671 | 1.3 × 106 | 1.2 × 105 | 1.9 × 103 | 4.5 × 104 | 5.9 × 106 |
| CHO | <1 | 1 | <0.3 | <2 | <2 | |
| CHOR16 | 5.4 × 104 | 4.7 × 103 | 9.8 × 101 | 9.7 × 102 | 2.8 × 105 | |
| NRK | <2 | 1.4 × 104 | <2 | <2 | <2 | |
| NRKR16 | 5.4 × 105 | 3.9 × 104 | 4.6 × 101 | 4.4 × 103 | 3.9 × 105 | |
lacZ(RD114) was produced by TELCeB6/RDF-7 helper-free packaging cells (7). lacZ(BaEV) was rescued by infection of mink Mv-1-Lu cells harboring a lacZ vector (35) with a replication-competent BaEV stock. Although MPMV could not package and rescue an MLV vector in the absence of MLV core proteins (28, 37), lacZ(MPMV) was produced by complementation of MLV core particles with MPMV Env; an MPMV env expression plasmid was constructed by replacing the RD114 env coding sequence in plasmid RDLF (7) with the MPMV env coding sequence derived from pTMOWT (4) (kindly provided by Eric Hunter) and transfected into TELCeB6 cells which express a lacZ vector and MLV Gag-Pol proteins (19a). lacZ(MPMV) was harvested from a pooled population of phleomycin-resistant transfectants. lacZ(SRV-1) and lacZ(SRV-2) were produced from TELCeB6 cells infected with SRV-1 and SRV-2, respectively, while infection of TE671/MFGnlslacZ cells lacking MLV Gag and Pol proteins by either SRV-1 or SRV-2 produced no pseudotype virus (data not shown). Titers are averages of two (experiment 2: RD114, BaEV, SRV-1, and SRV-2), three (experiment 1; RD114), or four (experiment 2; MPMV) infection studies. Titers of MPMV, BaEV, SRV-1, and SRV-2 were not determined in experiment 1, as indicated by dashes.
TE671 cells are human rhabdomyosarcoma cells. CHOR16 and NRKR16 cells are pooled populations of G418-resistant CHO and rat NRK cells, respectively, transfected with the R-receptor gene. 3T3R16 cells are NIH 3T3 cells trasduced with the R-receptor gene.