FIG 5.
Direct binding of CarR and CarRD55A to the upstream regions of the CarR regulon. (A to D) The 6-FAM-labeled DNA probes (5 nM) for the upstream regions of the eptA operon (A and C) and the carRS operon (B and D) were incubated with increasing amounts of CarR or CarRD55A as indicated. (A and B) For competition analysis, the same but unlabeled DNA probes were used as self-competitors, and various amounts of self-competitors were added to the reaction mixtures before the addition of CarR as indicated. B, bound DNA; F, free DNA.