FIG 6.

Specific and conserved binding sequences of CarR. (A and B) The 6-FAM-labeled DNA probes (40 nM) for the upstream regions of the eptA operon (A) and the carRS operon (B) were incubated with or without CarR (1000 nM) and then digested with DNase I. Each region protected by CarR is represented by a gray box. Nucleotides are numbered relative to the first base of the ORF for dgkA of the eptA operon (A) and carR of the carRS operon (B). (C) The sequences of the CarR-protected regions are aligned, and the consensus motif for the CarR binding is shown in red. The bases that match the consensus motif are shown in boldface.