FIG 7.

Assessment of direct effects of erythromycin exposure and erythromycin-associated gut microbiota effects on host physiology. Body weight over the 90-day study was assessed in (A) germ-free mice receiving erythromycin treatment or water and (B) germ-free mice colonized with erythromycin-associated or control microbiota. Cecum weight (C and E) and gut transit times (D and F) were also measured for the respective groups at the end of the 90-day study. Associations between the total gut transit time and cecal weight in (G) germ-free mice receiving erythromycin treatment or water and (H) recipients colonized with control or erythromycin-associated donor microbiota were determined using a linear regression model. Bar graphs and error bars represent the median and interquartile ranges, respectively. Between-group comparisons were statistically analyzed using a Mann-Whitney test, P < 0.05.