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Development of parasites after conditional PfFNT knockout. Giemsa staining of wild-type parasites (lower panel, DMSO) shows normal development from rings to schizonts after 4 d (two cycles). Parasites with conditional PfFNT knockout after rapamycin treatment (upper panel, RAP) stall at the trophozoite stage of cycle 1 and fail to progress to the next cycle, but gametocytes appear to develop normally.
