FIG 1.

Silencing efficiency of dsRNA targeting Met and EcR in A. stephensi. (A and B) Schematic representation of dsRNA fragments corresponding to the coding sequence (CDS) region of Met or EcR. T7 promoter was used to transcribe dsRNA. (C and D) Female A. stephensi adults were injected with dsMet or dsEcR, while dsRNA targeting GFP (dsGFP) served as a negative control. The RNAi efficiency was assessed in the carcass and gut of injected mosquitoes by qPCR at 3 days postinjection. The ribosome gene S7 was used as the reference gene. The expression values were normalized to dsGFP. The statistical significance was calculated using Student's t test. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.