Fig. 5.

Nucleolus organization in nas124. Top, TEM images of root cells from 10-day-old WT and nas124 plants grown in vitro. The Fibrillar Centers (FC) are surrounded by the Dense Fibrillar Component (DFC, indicated by white arrows) and embedded in the Granular Component (GC). Scale bar=500 nm. Bottom, boxplot graphs of nucleoli periphery (left) and number of FC structures per nucleolus (tight) in 5-, 10-, and 15-day-old root cells. The dots indicate the outlier samples. 28 (5-day-old), 13 (10-day-old), and 46 (15-day-old) WT nucleoli and 35 (5-day-old), 19 (10-day-old), and 16 (15-day-old) nas124 nucleoli were analysed. Asterisks indicate a significant difference calculated by Student’s t-test: ns P>0.05, ** P≤0.01, and **** P≤0.001.