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. 2023 May 16;74(15):4721–4735. doi: 10.1093/jxb/erad184

Fig. 4.

Fig. 4.

Role of the jasmonic acid pathway in accelerated HR in NbPLC3s-silenced plants. Empty vector control, NbPLC3s (PLC3s) and NbPLC3s:NbCoi1 double-knockdown (PLC3/coi1) plant leaves were infiltrated with R. solanacearum strain 8107. (A) Bacterial populations were determined in control, PLC3s, and PLC3/coi1 plants by plating at 0, 18, and 24 h following inoculation. Values are means ±SD of four replicate experiments. Asterisks show significant differences (P<0.05) between the control, NbPLC3s and double-knockdown plants. (B) Cell death induction was determined by measuring the ion conductivity levels in control, PLC3s, and PLC3s/coi1 plants. Values are means ±SD of four replicate experiments. (C) Total RNA was isolated from control, PLC3s, and PLC3s/coi1 plants at 0, 18, and 24 h after inoculation with R. solanacearum 8107. Expression values of Nbhin1 are shown as relative expression after normalization to internal standard genes (NbUbe35/NbNQO). Values represent means ±SD from triplicate experiments. Asterisks denote values significantly different from those of control plants (*; P<0.05, t-test).